State-of-the-art tube and plate luminometer “Made in Germany” by Titertek-Berthold guarantee high quality measurements in a wide range of glow and flash luminescent applications. Our luminometers are characterized by an outstanding sensitivity combined with unmatched flexibility in configuration and sample formats, to offer the ideal choice for every application.
Stand-alone models with excellent and easy-to-use protocols on board, as well as models driven by powerful Windows PC software allow the operation of different kinds of protocols including Kinetics and Dual Assay.
Validation tools for tube and plate luminometer complete the luminometer portfolio to ensure that the instruments meet the quality requirements.
Sirius II Tube Luminometer
Sirius L Tube Luminometer
Smart Line TL Tube Luminometer
Orion II Microplate Lumimometer
Luminescence-based methods feature excellent sensitivity, broad linear range and have no intrinsic background. Many luminescent methods are based on the fact that the oxidation of luciferin by the enzyme luciferase produces light, a reaction that needs ATP; but there is a broad range of other luminescent reactions than are used in laboratory methods:
Reporter genes are widely used in basic research, especially for the study of promoters. In these methods, the expression of a luciferase (typically firefly or renilla luciferase) is put under the control of the promoter of interest, allowing the detection of changes in the activity of the reporter by measuring the luminescence. Novel luciferases, such as the gaussia, metridia or cypridina luciferases feature higher sensitivity and are naturally secreted to the extracellular medium, allowing for convenient time-course experiments.
Chemiluminescence immunoassays (CLIA) are very similar to ELISA assays, but based on luminescence instead of absorbance, and have as a consequence better sensitivity and linear range. The source of light is usually a fast (“flash-type”) chemical reaction, normally based on acridinium esters or luminol, so the luminometers used to perform this kind of methods need to be equipped with one or more reagent injectors.
ATP assays: ATP is present in all living cells and is needed in the reaction of luciferin with luciferase to produce light, so it is not surprising that luminescence-based ATP assays are often used to quantify living cells, for example, to asses cell viability, cell proliferation or biomass. In addition to research, this kind of methods is very popular for hygiene monitoring.
The range of methods is broad and so is our range of luminometers. From low-priced tube luminometers to high-performance microplate luminometers suitable for high-throughput screening, we have the instrument you need.
If you are interested on automating your CLIAs or other assays, you may be interested in our assay workstation.